animal study · PMID 25738455

A mitochondrial-derived peptide, MOTS-c, acts as an insulin sensitizer in vivo — VialBase Research

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Last updated · 2015 · Lee, C., Zeng, J., Drew, B.G., Sallam, T., Martin-Montalvo, A., Wan, J., Kim, S.J., Mehta, H., Hevener, A.L., de Cabo, R., Cohen, P. · Cell Metabolism
Key findings
  • MOTS-c is the first mitochondrial-derived peptide shown to regulate metabolism
  • Prevented high-fat-diet-induced obesity and insulin resistance in mice
  • Activates AMPK pathway and enhances glucose metabolism

Summary

The discovery paper for MOTS-c as a metabolically active peptide. This Cell Metabolism publication identified MOTS-c as a novel mitochondrial-derived peptide (encoded within the 12S rRNA gene) that regulates metabolic homeostasis. Exogenous MOTS-c prevented obesity and insulin resistance in mice on a high-fat diet.

Key Findings

  • MOTS-c identified as a novel 16-amino-acid peptide encoded in mitochondrial DNA
  • Exogenous MOTS-c prevented high-fat-diet-induced obesity in mice
  • Improved insulin sensitivity and glucose tolerance
  • Activated AMPK signaling pathway — a key metabolic sensor
  • Enhanced glucose utilization and fatty acid oxidation
  • Represents a new class of signaling molecules: mitochondrial-derived peptides (MDPs)

Methodology

In-vitro studies in multiple cell lines showing MOTS-c effects on AMPK activation and glucose metabolism. In-vivo mouse studies: MOTS-c injection (IP) during high-fat diet feeding for up to 8 weeks. Metabolic phenotyping including glucose tolerance tests, insulin tolerance tests, body composition (DEXA), and metabolomics.

Limitations

  • Animal study (mice) — metabolic effects need human validation
  • Intraperitoneal injection in mice — optimal route for humans unknown
  • Chronic dosing effects and long-term safety not established
  • MOTS-c is mitochondrially encoded — implications for exogenous administration unclear
  • No dose-response characterization in this initial study

Relevance to Content

The foundational MOTS-c paper. Must-cite for any content about MOTS-c. Establishes the metabolic narrative: MOTS-c as a mitochondrial signal that regulates whole-body metabolism. The obesity prevention and insulin sensitization angles are directly relevant to the metabolic health audience. Connects to the broader “mitochondrial health” trend.

See Also